The Effects of GA3 and Organic Solvents on Acclimatization of Tissue Culture Propagated Black Cherry (Prunus serotina Ehrh.) Plantlets

Author: Kavanagh Kathleen L.   Lee Dong-Hun   Drew Allan P.   Maynard Charles A.  

Publisher: Society of American Foresters

ISSN: 0015-749X

Source: Forest Science, Vol.39, Iss.4, 1993-11, pp. : 644-654

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Abstract

Both gibberellic acid (GA3) and chilling were effective in inducing stem elongation of black cherry plantlets during acclimatization. At the rate of 100 mg/l, a foliar spray of GA3 was effective in stimulating stem elongation, but the effect was temporary. Most plantlets elongated to approximately 9 cm, but then set a resting bud. At rates above 200 mg/l, GA3 severely stunted root growth. An organic solvent solution enhanced the stem elongation response to GA3. When plantlets were treated with 100 mg/l GA3 in 0.2% DMSO and 54% ethanol, reinitiation of stem elongation was quicker, and bud set was delayed. Although the combination of GA3 and organic solvents induced stem elongation, it did not fully substitute for a chilling period. The minimum chilling period required to induce terminal bud break was 6 wk, and an additional 4 to 6 wk induced more rapid terminal bud break and stem elongation. For. Sci. 39(4): 644-654.

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