Author： Rajinimala Nataraj Rabindran Ramalingam Ramaiah Mathan
Publisher： Taylor & Francis Ltd
ISSN： 0323-5408
Source： Archives of Phytopathology and Plant Protection, Vol.42, Iss.6, 2009-06, pp. : 559-565
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Abstract
Serodiagnosis of BGYMV revealed that ACMV polyclonal antibody and ICMV monoclonal antibody trapped the particles from the extracts of BGYMV infected bitter gourd plants. Optimum antigen and ICMV monoclonal antibody dilution to detect BGYMV was 1:10 and 1:1, respectively. A405 value at 1.10 and 1:100 antigen dilution for BGYMV infected sample was 0.949 and 0.544, respectively. A405 value at 1:1 and 1:5 ICMV monoclonal antibody dilution was 0.993 and 0.696, respectively. Polymerase chain reaction technique using Deng's degenerate primer for gemini virus amplified 560 bp fragment from BGYMV infected bitter gourd plants.
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