A comparative analysis of total serum miRNA profiles identifies novel signature that is highly indicative of metastatic melanoma: a pilot study

Author： Greenberg Eyal Besser Michal J. Ben-Ami Eytan Shapira-Frommer Ronnie Itzhaki Orit Zikich Dragoslav Levy Daphna Kubi Adva Eyal Eran Onn Amir Cohen Yehudit Barshack Iris Schachter Jacob Markel Gal

Publisher： Informa Healthcare

ISSN： 1366-5804

Source： Biomarkers, Vol.18, Iss.6, 2013-09, pp. : 502-508

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Abstract

AbstractContext: Quantification of circulating microRNAs (miRNAs) has recently become feasible and reliable, with most efforts focusing on miRNAs overexpressed by cancer cells.Objective: Identification of a characteristic circulating miRNAs profile in melanoma patients.Methods: We conducted a pilot study comprised of unbiased qPCR comparison of serum miRNA profiles between metastatic melanoma patients and healthy donors.Results: Loss of two normal serum-miRNAs, miR-29c and miR-324-3p, is highly indicative of metastatic melanoma. Hierarchical clustering analysis supported the results and clearly distinguished melanoma patients from healthy donors, metastatic colon and renal cancer patients.Discussion and conclusions: This approach is independent of tumor heterogeneity and is expected to have superior biomarker performances.