Transplantation of M213-2O Cells with Enhanced GAD₆₇ Expression into the Inferior Colliculus Alters Audiogenic Seizures

Author： Ross K.C. Waldman B.C. Conejero-Goldberg C. Freed W. Coleman J.R.

ISSN： 0014-4886

Source： Experimental Neurology, Vol.177, Iss.1, 2002-09, pp. : 338-340

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Abstract

The purpose of the present study was to examine the effects of GABA-producing cell transplants on audiogenic seizures (AGS). The M213-2O cell line was derived from fetal rat striatum and has GABAergic properties. This cell line was further modified to express human GAD₆₇ and produce elevated levels of GABA. The present study compares the effects of parent M213-2O cell transplants with those of GAD₆₇-modified M213-2O cells in AGS-prone Long–Evans rats. Two weeks following implantation of engineered cells, latency to AGS-typical wild running was increased compared to nonimplanted subjects. Survival of the transplanted cells was confirmed by immunochemical labeling of GAD₆₇ and Epstein–Barr virus nuclear antigen. These findings support the use of GABA-producing cell lines to modify seizure activity. © 2002 Elsevier Science (USA).