消息
loading
Selective Blockade of AT 1 Angiotensin II Receptors Abolishes Ischemic Preconditioning in Isolated Rabbit Hearts

Author: Diaz R.J.   Wilson G.J.  

Publisher: Academic Press

ISSN: 0022-2828

Source: Journal of Molecular and Cellular Cardiology, Vol.29, Iss.1, 1997-01, pp. : 129-139

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

The aim of the present study was to assess the participation of angiotensin II receptors in the triggering mechanism of ischemic preconditioning. Isolated buffer-perfused rabbit hearts were subjected to 40 min of regional ischemia (37#°C) followed by 60 min of reperfusion. Ischemic preconditioning was induced with three cycles of 5-min ischemia and 10-min reperfusion given prior to the 40-min ischemic period. Infarct size and ventricular function were assessed. Ischemic preconditioning reduced infarct size to 5.2±1.2% of the area at risk (mean± s.e.m. , P <0.001) when compared to controls (26.4±3.0%), but did not protect against ventricular dysfunction. Activation of angiotensin II receptors with angiotensin II (100 n m ) also limited infarct size (9.6±2.2%, P <0.01 v control group). Inhibition of angiotensin II receptors with [Sar 1 , Val 5 , Ala 8 ]-angiotensin II (saralasin, 1 & mu; m ) blocked the protection of ischemic preconditioning against necrosis (29.7±3.2%) while it did not increase infarct size in saralasin-treated control hearts (31.5±3.9%). Furthermore, inhibition of the AT 1 subtype of the angiotensin II receptor with losartan (20 & mu; m ), but not inhibition of the AT 2 subtype with PD-123,319 ditrifluoroacetate (10 & mu; m ), abolished the infarct size-limiting effect of ischemic preconditioning. We conclude that the AT 1 angiotensin II receptor participates in ischemic preconditioning. Thus, in the isolated rabbit heart, activation of AT 1 receptors must occur before prolonged ischemia for ischemic preconditioning to limit infarction.

Related content