Author： Ankri S. Reyes O. Leblon G.
Publisher： Academic Press
ISSN： 0147-619X
Source： Plasmid, Vol.35, Iss.1, 1996-01, pp. : 62-66
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Abstract
Highly DNA-restrictive Corynebacteria can be transformed with DNA made in vitro by PCR amplification of a sequence that contains the replication origin of pBL1, a plasmid common to many Corynebacteria. In all strains examined, the transformation efficiencies of PCR-synthetized DNA equal or improve the performances of heterologous DNA extracted from wild-type and dam - -dcm - strains of Escherichia coli. The transformation efficiencies obtained with PCR-made DNA may be high enough to permit its general application to experiments of gene integration.
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