Author: Jeung J.U. Cho S.K. Shim K.S. Ok S.H. Lim D.S. Shin J.S.
Publisher: Academic Press
ISSN: 0147-619X
Source: Plasmid, Vol.48, Iss.2, 2002-09, pp. : 160-163
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Abstract
For applications such as sequencing, transfection, and in vitro transcription, PCR products have to be subcloned into plasmids. Many strategies are used for cloning, blunt-end ligation or the incorporation of restriction endonuclease sites into PCR primers for appropriate vectors. However, the most convenient and direct method is T/A cloning. In this study, we developed two of the pGEM-7Zf(+) phagemid T-tail vectors using
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