Green fluorescent protein as a reporter in the Brassica–Leptosphaeria maculansinteraction

Author： Sexton A.C.   Howlett B.J.  

Publisher： Academic Press

ISSN： 0885-5765

Source： Physiological and Molecular Plant Pathology, Vol.58, Iss.1, 2001-01, pp. : 13-21

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

Previous Menu Next

Abstract

Leptosphaeria maculans, the fungus which causes blackleg of oilseed Brassica spp., was transformed with a gene encoding the reporter molecule Green Fluorescent Protein (GFP). The Aspergillus nidulans gpdA promoter was fused to the GFP gene to enable constitutive expression, and this was used to follow disease progression. Also, the GFP gene was fused to the L. maculans promoter for cyanide hydratase (Cht), a fungal enzyme that catalyses the detoxification of cyanide. Northern analyses and fluorescence image analyses of L. maculans transformed with this construct confirmed that GFP transcription occurred under similar conditions to that of Cht. GFP was expressed from the Cht promoter in cotyledon lesions on Brassica napus and Brassica juncea, and the high level of fluorescence in stem lesions indicated that Cht is also transcribed during infection of the stem.

Related content

Differences in ozone sensitivity in three varieties of cabbage (Brassica oleracea L.) in the rural Mediterranean area

By Calatayud A.   Alvarado J.W.   Barreno E.  

Journal of Plant Physiology, Vol. 159, Iss. 8, 2002-08 ,pp. : 863-868 (6)

Urban & Fischer

Access to resources Recommend Favorite

Changes in the wheat Em 5′ UTR affect reporter gene expression in vivo and in vitro

By Paradkar M.R.   Marcotte Jr. W.R.  

Journal of Plant Physiology, Vol. 158, Iss. 7, 2001-07 ,pp. : 929-934 (6)

Urban & Fischer

Access to resources Recommend Favorite

In planta monitoring of the activity of two constitutive promoters, CaMV 35S and TR2′, in developing feeding cells induced by Globodera rostochiensis using green fluorescent protein in combination with confocal laser scanning microscopy

By Goverse A.   Biesheuvel J.   Wijers G.   Gommers F.J.   Bakker J.   Schots A.   Helder J.  

Physiological and Molecular Plant Pathology, Vol. 52, Iss. 4, 1998-03 ,pp. : 275-284 (10)

Academic Press

Access to resources Recommend Favorite

Modelling Nitrogen Content and Distribution in Cauliflower (Brassica oleracea L.botrytis)

By Alt C.   Kage H.   Stützel H.  

Annals of Botany, Vol. 86, Iss. 5, 2000-11 ,pp. : 963-973 (11)

Oxford University Press

Access to resources Recommend Favorite

Nucleotide and amino acid variation in the coat protein coding region of turnip mosaic virus isolates and possible involvement in the interaction with the brassica resistance gene TuRB01

By Lehmann P.   Petrzik K.   Jenner C.   Greenland A.   Špak J.   Kozubek E.   Walsh J.A.  

Physiological and Molecular Plant Pathology, Vol. 51, Iss. 3, 1997-09 ,pp. : 195-208 (14)

Academic Press

Access to resources Recommend Favorite