Genomic Structure and Chromosomal Mapping of the Nuclear Orphan Receptor RORgamma (RORC) Gene

Author： Medvedev A. Chistokhina A. Hirose T. Jetten A.M.

ISSN： 0888-7543

Source： Genomics, Vol.46, Iss.1, 1997-11, pp. : 93-102

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Abstract

The nuclear orphan receptor subfamily ROR/RZR is part of the steroid and thyroid hormone/retinoid receptor superfamily and consists of three different genes, alpha, beta, and gamma. In this study, we determined the genomic structure of mouse RORgamma and the chromosomal localization of both mouse RORgamma and human RORgamma (HGMW-approved symbol RORC). The genomic structure of the mouse RORgamma gene was derived from the analysis of P1 vector clones containing large genomic fragments encoding RORgamma. These results revealed that the mRORgamma gene has a complex structure consisting of 11 exons separated by 10 introns spanning more than 21 kb of genomic DNA. The DNA-binding domain is contained in two exons, 3 and 4, each encoding one zinc-finger. The splice site between exon 3 and exon 4 is identical to that found in RAR and TR3 receptors. RORgamma is expressed as two mRNAs, 2.3 and 3.0 kb in size, that are derived by the use of alternative polyadenylation signals. We show by fluorescence in situ hybridization that the mouse RORgamma gene is located on chromosome 3, in a region that corresponds to band 3F2.1-2.2. The human RORgamma was mapped to chromosome region 1q21. The results demonstrate that the RORgamma genes are located in chromosomal regions that are syntenic between mouse and human. Copyright 1997 Academic Press.