Author： Kong S. Heel K.A. Hall J.C. McCauley R.D.
Publisher： Academic Press
ISSN： 0890-8508
Source： Molecular and Cellular Probes, Vol.12, Iss.5, 1998-10, pp. : 339-341
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Abstract
The concentration of mRNA within the intestinal mucosa is usually measured by either Northern blot analysis or semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). However, these methods are limited by a lack of valid internal controls, low sensitivity, and large differences in the concentration of the internal control and target gene. The authors present an alternative method using competitive RT-PCR to measure glutaminase mRNA in isolated enterocytes.
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