Author: Christensen T. Bruhn T. Balchen T. Diemer N.H.
Publisher: Academic Press
ISSN: 0969-9961
Source: Neurobiology of Disease, Vol.1, Iss.3, 1994-12, pp. : 131-138
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Abstract
Systemic administration of salicylate (SA) to rats (100 mg kg -1 i.p.) was used as an in vivo trap of hydroxyl radicals ( · OH). In the brain SA reacts with hydroxyl radicals to form the stable adducts 2,3- and 2,5 dihydroxybenzoic acid (DHBAs) which can thus be taken as an index of · OH formation. The DHBAs were recovered by intracerebral microdialysis in hippocampus or striatum and quantified by high pressure liquid chromatography (HPLC) with electrochemical detection. There were no peaks corresponding to 2,5-DHBA or 2,3-DHBA in the chromatograms from rats not receiving SA. A basal level of 2,5-DHBA was seen in the dialysates from all animals given SA whereas 2,3-DHBA was not detected. In one group of rats generation of free oxygen radicals was induced in the striatum by adding Fe 2+ and ascorbate to the perfusion fluid to test the sensitivity of the system. Addition of Fe 2+ ascorbate to the perfusion fluid induced a significant 7-fold increase in 2,5-DHBA that gradually returned to baseline after removal of Fe 2+ /ascorbate. In two other groups the microdialysis probes were implanted in either the striatum or the hippocampus and the animals were subjected to 20 min of four-vessel occlusion + hypotension (4-VOH). Significant reductions in 2,5-DHBA were detected during ischaemia followed by significant increases of 5-fold and 3-fold in the striatum and hippocampus, respectively, beginning immediately upon reperfusion and lasting for the remainder of the observation period (160 min).
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