Modification to Intestinal Glycosidase Activities Following Bifidobacterium breve C50 Oral Challenge in C3H Mice

Author： Mullié C. Romond M.B. Yazourh A. Bezirtzoglou E. Romond C.

ISSN： 1075-9964

Source： Anaerobe, Vol.5, Iss.3, 1999-03, pp. : 499-504

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Abstract

The hypothesis that consumption of bifidobacteria increases intestinal bifidobacteria, decreases pH and changes glycosidase balance was examined in conventional mice. A commercially available strain of bifidobacteria,Bifidobacterium breve C50, was tracked 2, 4 and 6 h post-challenge through the gastrointestinal tract of mice. One group (naive mice) consumed the strain for the first time. The other mice were accustomed over 15 days to a daily intake of 10⁷–10⁸cfu/mL. Bifidobacterium breve C50 was not isolated throughout the intestine. After a 15 day-intake, a decrease in endogenous bifidobacteria occured, which was not expected in the distal ileum. This suggested an inhibitory effect induced by the probiotic strain. Post-challenge, naive mice exhibited a fast increase in colonic bifidobacterial counts, whereas adapted mice showed no increase in colonic bifidobacteria but mucus was acidified. This contrasted with the overall increase in pH seen post-challenge in naive mice. In addition, all cecal glycosidases were expressed at a lower level after a first administration. Only caecal acid galactosidase activity decreased post-challenge in adapted mice. In conclusion, data suggested how difficult it is to alter intestinal bifidobacteria permanently and to affect physiological function by feeding bifidobacteria.