Transverse-Relaxation-Optimized (TROSY) Gradient-Enhanced Triple-Resonance NMR Spectroscopy

Author: Loria J.P.   Rance M.   Palmer A.G.  

Publisher: Academic Press

ISSN: 1090-7807

Source: Journal of Magnetic Resonance, Vol.141, Iss.1, 1999-11, pp. : 180-184

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Abstract

Two modifications to sensitivity-enhanced gradient-selected TROSY-based triple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-flipback solvent suppression. The modifications are illustrated for the HNCO–TROSY experiment, but are applicable to all triple-resonance experiments that detect proton magnetization after a reverse polarization transfer step from a 15N spin. The methods are applied to yeast triosephosphate isomerase, a symmetric dimer with 248 amino acid residues per monomer.