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Cosine Modulated HSQC: A Rapid Determination of ³J_HNHα Scalar Couplings in ¹⁵N-labeled Proteins

Author： Petit A. Vincent S.J.F. Zwahlen C.

Publisher： Academic Press

ISSN： 1090-7807

Source： Journal of Magnetic Resonance, Vol.156, Iss.2, 2002-06, pp. : 313-317

Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.

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Abstract

A two-dimensional HSQC-based NMR method, ¹⁵N-COSMO-HSQC, is presented for the rapid determination of homonuclear ³J_HNHα couplings in ¹⁵N-labeled proteins in solution. Scalar couplings are extracted by comparing the intensity of two separate datasets recorded with and without decoupling of the ³J_HNHα during a preparation period. The scalar couplings are introduced through a cosine modulation of the peak intensities. The experiment relies on a BIRD sandwich to selectively invert all amide protons H^N and is very simple to implement. ³J_HNHα couplings were determined using both the ¹⁵N-COSMO-HSQC and quantitative-J on ¹⁵N-labeled chemokine RANTES. The two experiments show well-correlated values. © 2002 Elsevier Science (USA).

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