Accumulation of FoxP3-expressing CD4+CD25+ T cells with distinct chemokine receptors in synovial fluid of patients with active rheumatoid arthritis

Author: Jiao Z.   Wang W.   Jia R.   Li J.   You H.   Chen L.   Wang Y.  

Publisher: Informa Healthcare

ISSN: 0300-9742

Source: Scandinavian Journal of Rheumatology, Vol.36, Iss.6, 2007-01, pp. : 428-433

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Abstract

Objective: To explore the presence and characteristics of FoxP3-expressing CD4+CD25+ regulatory T cells in synovial fluid (SF) of patients with active rheumatoid arthritis (RA). Methods: The frequency and chemokine receptors expression profile of FoxP3-expressing CD4+CD25+ regulatory T cells in SF and peripheral blood (PB) from RA patients and PB from healthy controls were investigated by flow cytometry using three- or four-colour intracellular staining. Results: The frequency of CD4+CD25+ FoxP3+ T cells was increased significantly in SF compared with paired PB from RA patients and PB from healthy controls (p<0.05). However, the frequency in PB from RA patients was significantly lower than in PB from healthy controls (p<0.05). Notably, CD4+CD25+FoxP3+ T cells in SF expressed increased levels of inflammation-related trafficking chemokine receptors, such as CCR4, CCR5, and CXCR4. Conclusion: There is an accumulation of FoxP3-expressing regulatory T cells in RA SF, and such recruitment may be dependent on the distinct chemokine receptors expressed on regulatory T cells.

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