In-Vitro Effects of Dexamethasone on Cellular Proliferation, Apoptosis, and Na+-K+-ATPase Activity of Bovine Corneal Endothelial Cells

Author: Chen Wei-Li   Lin Chung-Tien   Yao Chung-Chen   Huang Yu-Hua   Chou Yu-Bin   Yin Hsiang-Shu   Hu Fung-Rong  

Publisher: Informa Healthcare

ISSN: 0927-3948

Source: Ocular Immunology and Inflammation, Vol.14, Iss.4, 2006-07, pp. : 215-223

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Abstract

Purpose : To assess the in-vitro effects of dexamethasone (DEX) on the proliferation, apoptosis, and Na+-K+-ATPase activity of bovine corneal endothelial cells. Methods : Bovine corneal endothelial cells were cultured with DEX ranging from 10 −10 to 10 −3 M. The effect of DEX on the proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay. Apoptosis and necrosis were detected by staining with fluorescein-conjugated annexin V and propidium iodide, followed by flow cytometry. The effect of DEX on Na+-K+-ATPase activity was evaluated using non-isotopic methods. Results : DEX did not affect cellular proliferation or induce apoptosis/necrosis from 10 −10 to 10 −5 M. At 10 −4 and 10 −3 M, DEX significantly decreased proliferation and increased apoptosis and/or necrosis. DEX significantly increased the Na+-K+-ATPase activity from 10 −8 to 10 −6 M, with the maximal effect at 10 −6 M ( p

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