Author: Chen Wei-Li Lin Chung-Tien Yao Chung-Chen Huang Yu-Hua Chou Yu-Bin Yin Hsiang-Shu Hu Fung-Rong
Publisher: Informa Healthcare
ISSN: 0927-3948
Source: Ocular Immunology and Inflammation, Vol.14, Iss.4, 2006-07, pp. : 215-223
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Purpose : To assess the in-vitro effects of dexamethasone (DEX) on the proliferation, apoptosis, and Na+-K+-ATPase activity of bovine corneal endothelial cells. Methods : Bovine corneal endothelial cells were cultured with DEX ranging from 10 −10 to 10 −3 M. The effect of DEX on the proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay. Apoptosis and necrosis were detected by staining with fluorescein-conjugated annexin V and propidium iodide, followed by flow cytometry. The effect of DEX on Na+-K+-ATPase activity was evaluated using non-isotopic methods. Results : DEX did not affect cellular proliferation or induce apoptosis/necrosis from 10 −10 to 10 −5 M. At 10 −4 and 10 −3 M, DEX significantly decreased proliferation and increased apoptosis and/or necrosis. DEX significantly increased the Na+-K+-ATPase activity from 10 −8 to 10 −6 M, with the maximal effect at 10 −6 M ( p
Related content
By Weng C-F. Zhao W. Fegeding K.V. Komisar J.L. Tseng J.
International Immunology, Vol. 11, Iss. 5, 1999-05 ,pp. :
By Wang Yafei Ghali Wa-el Pingle Pavani Traboulsi Amar Dalal Tajas O'Rourke James Cone Robert E.
Ocular Immunology and Inflammation, Vol. 11, Iss. 1, 2003-03 ,pp. :
By Liu H.-C. Anday J.K. House S.D. Chang S.L.
Journal of Neuroimmunology, Vol. 146, Iss. 1, 2004-01 ,pp. :