Acetyl esterase from mediterranean oranges: partial purification, immobilisation and biotransformations

Author: Pasta Piero   Verga Roberto   Zambianchi Francesca   Daminati Moreno  

Publisher: Informa Healthcare

ISSN: 1024-2422

Source: Biocatalysis and Biotransformation, Vol.22, Iss.3, 2004-05, pp. : 221-224

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Abstract

Acetyl esterase (acetic-ester acetylhydrolase, EC 3.1.1.6) from citrus peel, whose natural role is not well known, catalyses, in vitro, the hydrolysis of acetyl groups from a wide range of substrates. This enzyme was extracted from Mediterranean orange peel, largely available in Italy, and purified 190-fold by a single chromatographic step on Sepabeads FP-HG. SDS polyacrylamide gel electrophoresis of the purified enzyme showed a major protein band, corresponding to a molecular mass of 45 kDa. Both free and immobilised enzyme were used in biotransformations. The enzyme removed the acetyl group in the 3 position of β-lactamic antibiotics, such as cephalosporin C and the intermediate 7-aminocephalosporanic acid with ≥98% conversion and 91-93% product yield.

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