Effect of Peroxynitrite Scavenging on Endothelial Cells Stimulated by Plasma from Women with Preeclampsia: A Proteomic Approach

Author： Sankaralingam Sowndramalingam   Lalu Manoj M.   Xu Yi   Davidge Sandra T.  

Publisher： Informa Healthcare

ISSN： 1064-1955

Source： Hypertension in Pregnancy, Vol.29, Iss.4, 2010-11, pp. : 419-428

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Abstract

Hypothesis: Increased peroxynitrite formation in endothelial cells incubated with plasma from women with preeclampsia alters the expression of specific endothelial proteins. Methods: We used a proteomic approach to examine the effect of a peroxynitrite scavenger (FeTPPS, 5 μmol/L) on the expression of endothelial proteins in response to plasma from women with preeclampsia. After treatment, cells were subjected to two-dimensional gel electrophoresis and proteins were identified using mass spectrometry. Results: Peroxynitrite scavenging decreased the expression of structural proteins, cytokeratin, actin-γ-1, and moesin, and the calcium-binding protein, calumenin. Conclusions: This study has identified novel proteins that are regulated by peroxynitrite in response to plasma from women with preeclampsia.

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