Characterisation of a cDNA encoding γ-glutamylcysteine synthetase in Medicago truncatula

Author: Frendo Pierre   Mathieu Christel   Van de Sype Ghislaine   Hérouart Didier   Puppo Alain  

Publisher: Informa Healthcare

ISSN: 1071-5762

Source: Free Radical Research, Vol.31, Iss.1, 1999-01, pp. : 213-218

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Abstract

A γ-ECS cDNA from Medicago truncatula was isolated using an Arabidopsis thaliana cDNA as probe. The analysis of the amino acid sequence deduced from this cDNA revealed 80% identity with the γ-ECS from A. thaliana and Brassica juncea and suggested a plastidial localisation for the enzyme. γ-ECS activity and high level of GSH were detected in the γ-ECS-deficient E. coli strain expressing a fusion protein containing the M. truncatula γ-ECS protein. Southern blot analysis suggests that γ-ECS is encoded by a small multigenic family in M. truncatula and shows that homologous genes are present in two other leguminous plants, Medicago sativa and Pisum sativum . γ-ECS gene expression was analysed by Northern blot in seedlings, plantlets and mature plants.

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