Reduction in free-radical-induced DNA strand breaks and base damage through fast chemical repair by flavonoids

Author: Anderson Robert   Amarasinghe Chanchala   Fisher Louisa   Mak Wai   Packer John  

Publisher: Informa Healthcare

ISSN: 1071-5762

Source: Free Radical Research, Vol.33, Iss.1, 2000-01, pp. : 91-103

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Abstract

This paper provides evidence that dietary flavonoids can repair a range of oxidative radical damages on DNA, and thus give protection against radical-induced strand breaks and base alterations. We have irradiated dilute aqueous solutions of plasmid DNA in the absence and presence of flavonoids (F) in a “constant · OH radical scavenging environment”, k of 1.5 × 10 7 s -1 by decreasing the concentration of TRIS buffer in relation to the concentration of added flavonoids. We have shown that the flavonoids can reduce the incidence of single-strand breaks in double-stranded DNA as well as residual base damage (assayed as additional single-strand breaks upon post-irradiation incubation with endonucleases) with dose modification factors of up to 2.0 ± 0.2 at [F]