Author: Schild Lorenz Makarow Petr Haroon Fahad Krautwald Karla Keilhoff Gerburg
Publisher: Informa Healthcare
ISSN: 1071-5762
Source: Free Radical Research, Vol.42, Iss.3, 2008-02, pp. : 237-243
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
A solid tumour undergoes ischemia/reperfusion due to deficient vascularization and subsequent formation of new blood vessels. This study investigated the effect of transient oxygen and glucose deprivation (OGD) on proliferation of C6 glioma cells. The cells were subjected to 18 h of OGD followed by reoxygenation in the presence of glucose and different extra-cellular H2O2 concentrations since H2O2 affects cell proliferation. After reoxygenation, the cellular H2O2 concentration was increased returning to control levels within 24 h. Within this period, increase in cell number and MTT-reduction were impaired. Regeneration was completed on the third day of reoxygenation. MTT-reduction increased faster than cell number, indicating an OGD-dependent up-regulation of protein expression. It is concluded that ischemia/reperfusion stress promotes proliferation of tumour cells. An essential factor is a distinct H2O2 concentration. Massive elevation as well as significant reduction of H2O2 concentration impairs the proliferation process.
Related content
By He Quan-Wei Xia Yuan-Peng Chen Sheng-Cai Wang Yong Huang Ming Huang Yan Li Jian-Yong Li Ya-Nan Gao Yuan Mao Ling Mei Yuan-Wu Hu Bo
Molecular Neurobiology, Vol. 47, Iss. 3, 2013-06 ,pp. :
By Xu Feng Yu Haixia Liu Jinyao Cheng Lu
Molecular and Cellular Biochemistry, Vol. 386, Iss. 1-2, 2014-01 ,pp. :
By de Pablo Yolanda Nilsson Michael Pekna Marcela Pekny Milos
Histochemistry and Cell Biology, Vol. 140, Iss. 1, 2013-07 ,pp. :