Author： Harris John W.
Publisher： Informa Healthcare
ISSN： 1362-3095
Source： International Journal of Radiation Biology, Vol.11, Iss.5, 1966-05, pp. : 465-476
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Abstract
The response of isolated leucocyte lysomes to gamma-radiation and to heat was directly related to the methods of preparation used. Lysosomes prepared with minimal damage were relatively sensitive to radiation damage; effects were observed after doses as low as 200 rads. Such lysosomes, in contrast to those prepared by more vigorous methods, were relatively stable during incubation at 28°c and were highly susceptible to mechanical damage. Addition of ions to the suspending medium resulted in an immediate increase in optical density and reduction in the turbidity changes and enzyme release during subsequent incubation. The extent of these effects varied with different ions and was not due to changes in the osmotic pressure of the medium. The possible relationship between these results and the wide range of radiosensitivity reported for lysosomes isolated from various tissues was discussed.
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