The Effect of Post-irradiation Centrifugation on Chromosome Aberrations in Vicia Faba

ISSN： 1362-3095

Source： International Journal of Radiation Biology, Vol.15, Iss.6, 1969-09, pp. : 519-523

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Abstract

Vicia faba primary roots were x-irradiated in air from 0-700 R and immediately centrifuged for 20 min (2500g) at room temperature. Metaphase analysis of chromosome aberrations showed that with centrifugation there are: (1) a small but consistent increase in chromosome exchanges (two-break aberrations); (2) no significant difference between the centrifugation enhancement ratios for exchanges at the various x-ray doses of the dose-response curves; (3) no increase in terminal deletions (one-break aberrations); (4) no consistent increase in interstitial deletions (ID). The results are consistent with the hypothesis that this post-irradiation treatment increased the number of sites in the interphase nucleus where chromosomes are close enough to exchange if broken by x-rays.

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