Water-derived Radicals and Radiation Sensitivity of Bacteriophage T7

Author: Powers E.L.   Gampel-Jobbagy Zulema  

Publisher: Informa Healthcare

ISSN: 1362-3095

Source: International Journal of Radiation Biology, Vol.21, Iss.4, 1972-04, pp. : 353-359

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Abstract

The coliphage T7 was x-irradiated in the presence and absence of ethanol, an effective ·OH and ·H scavenger, in buffered suspensions saturated with N2, N2O and O2. In 10−3 × dilution of the original suspending organic medium, ethanol was protective in all three experimental conditions. In 10−5 × dilution of the original medium (equivalent to contaminant free), ethanol is also protective in all three experiments and to a greater extent; but the relative order of radiation sensitivities changes. It is concluded that, when irradiated in pure suspension, T7 phage is inactivated partly by ·OH, and that ·H and eaq play small, if any, roles as inactivating species. Additional observations were that the 'O2-protection' phenomenon absent in purified suspension is observed in 10−3 M ethanol solutions; and that there is evidence that exhaustion of additives and, perhaps, build-up of products are responsible for the biphasic natures of some response lines. Also, there is evidence that radiation-induced sub-lethal damage to the phage particles may be important in determining sensitivity.