Author： Redpath J.L. Santus R. Ovadia J. Grossweiner L.I.
Publisher： Informa Healthcare
ISSN： 1362-3095
Source： International Journal of Radiation Biology, Vol.28, Iss.3, 1975-09, pp. : 243-253
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Abstract
The radiation-induced inactivation of dilute solutions of three forms of bovine carbonic anhydrase (metal-free apo-BCA, Zn2+-BCA and Co2+-BCA) has been studied. The presence of the metal ions did not alter the sensitivity of the enzyme to inactivation by oxidizing radicals; however, they exerted a protective effect against inactivation by reducing radicals. Data obtained using the amino-acid-specific inorganic radical anions (CNS)2− and Br2− allowed the identification of histidine, tyrosine and tryptophan as residues essential to the activity of bovine carbonic anhydrase when assayed by p-nitro-phenylacetate.
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