Heterogeneity in Radiosensitization by Heat of Cloned Cell Lines Derived from a Single Human Melanoma Xenograft

ISSN： 1362-3095

Source： International Journal of Radiation Biology, Vol.50, Iss.1, 1986-07, pp. : 93-102

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Abstract

Heterogeneity in radiosensitization by heat was studied using one uncloned and five cloned cell lines isolated from a single tumour of a human melanoma xenograft. Cells from passages 7-12 in vitro were given heat treatments of 42·5°C (45 min), 43·5°C (45 min) or 44·5°C (45 min) immediately after exposure to graded doses of radiation. The survival curves after irradiation alone had similar D₀ values but differed in the size of the shoulder. The heterogeneity in heat radiosensitization was reflected in differences in decrease of the D₀ values. The thermal enhancement ratios, calculated from the D₀ values, were in the ranges 1·2 ± 0·2-1·7 ± 0·2 (42·5°C), 1·4 ± 0·3-2·4 ± 0·4 (43·5°C) and 2·3 ± 0·4-3·4 ± 0·4 (44·5°C). Moreover, at 43·5°C the heterogeneity was also reflected in different modifications of the shape of the survival curves. Two lines showed survival curves with a significant shoulder and a relatively low D₀ value whereas two other lines had lost the shoulder almost completely but showed relatively high D₀ values. All lines showed survival curves with a broad shoulder after heating at 42·5°C, whereas none of the lines showed survival curves with a significant shoulder after heating at 44·5°C.

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