Author: Dulon D. Zajic G. Schacht J.
Publisher: Informa Healthcare
ISSN: 1362-3095
Source: International Journal of Radiation Biology, Vol.55, Iss.6, 1989-06, pp. : 1007-1014
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Abstract
Living outer hair cells were irradiated under conditions of fluorescent microscopy (epi-illumination through the objective) with UVR (waveband 340-380 nm), blue light (waveband 450-490 nm) or green light (waveband 515-560 nm) in the intracellular presence or absence of the fluorescent dyes fura-2 or 2′,7′-bis-(carboxyethyl) 5-(and 6-)carboxyfluorescein (BCECF). In response to UVR with or without intracellular fura-2 and to blue light in the presence of BCECF (irradiation intensities of 2−12 × 105 W/m2), the cells shortened and swelled within 15-30 s, accompanied by the formation of numerous cytoplasmic granulations. The cellular reactions were significantly delayed to 3 min by the addition of 1 mm of the radical scavengers