Author： Tamba M. Torreggiani A.
Publisher： Informa Healthcare
ISSN： 1362-3095
Source： International Journal of Radiation Biology, Vol.74, Iss.3, 1998-09, pp. : 333-340
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Abstract
Purpose: To investigate the potential antioxidative role of carnosine (beta -alanyl-L-histidine) through its interaction with radiation induced radicals. Materials and methods: Pulse radiolysis experiments were performed by a 12MeV electron linear accelerator (LINAC) on carnosine aqueous solutions at different pHs. The Raman spectra of solid samples were obtained by a Bruker IFS 66 spectrometer. Results: The protonation state of the imidazole ring was observed to affect the site(s) of OH attack on carnosine as well as the decay rates of the resulting adducts. Reasonably, a base catalysed water elimination from the adducts leading to a resonancestabilized radical, which absorbs at lambda 270nm, takes place. Raman spectra in slightly alkaline medium have indicated that carnosine is mainly present as tautomer I and, of consequence, positions C(2) and C(4) of the imidazole ring are the preferential sites for OH attack. Conclusions: These studies have shown that carnosine is a good scavenger of OH radicals giving rise to a quite stable intermediate which should be less reactive towards other biological components than OH itself. Raman data have been helpful in predicting the preferential sites for the OH attack.
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