Quantification of ultraviolet radiation-induced DNA damage in the urine of Swedish adults and children following exposure to sunlight

Author： Liljendahl Tove Sandberg Kotova Natalia Segerbäck Dan

Publisher： Informa Healthcare

ISSN： 1366-5804

Source： Biomarkers, Vol.17, Iss.7, 2012-11, pp. : 634-641

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Abstract

Context: DNA damage following exposure to ultraviolet radiation (UVR) is important in skin cancer development. The predominant photoproduct, cyclobutane thymine dimer (T=T), is repaired and excreted in the urine, where it provides a biomarker of exposure.Objective: To quantify urinary T=T levels after recreational sunlight exposure in adults and children.Methods: Average UVR doses were measured with personal dosimeters. Urinary T=T was analysed with ³²P-postlabelling.Results: Background levels of T=T increased significantly following exposure to sunlight. Amounts of T=T in urine of children and adults were not significantly different after adjusting for area of skin exposed and physiological differences. UVR dose and amounts of T=T correlated for both adults and children.Conclusion: Recreational exposure to sunlight in Sweden induces levels of DNA damage, clearly detectable in urine.