Expression of smooth muscle calponin in synovial sarcoma

Author： Ono Hidefumi Yoshikawa Hideki Ueda Takafumi Yamamura Hisako Kudawara Ikuo Manou Masayuki Ishiguro Shingo Funai Hiroko Koyanagi Yumiko Araki Nobuhito Hashimoto Nobuyuki Sonobe Hiroshi Tatsuta Masaharu Takahashi Katsuhito

Publisher： Informa Healthcare

ISSN： 1369-1643

Source： Sarcoma, Vol.3, Iss.2, 1999-09, pp. : 107-113

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Abstract

Purpose. Histogenesis of synovial sarcoma remains controversial and reliable molecular markers for diagnosis are necessary. Expression of basic calponin, a smooth muscle differentiation-specific actin-binding protein, was studied in synovial sarcoma. Subjects and Methods. The basic calponin gene and the gene product were analyzed by reverse transcription PCR analysis (RT-PCR) and immunohistochemistry in 14 synovial sarcomas and a human synovial sarcoma cell line (HS-SY-II). Results and Discussion. Immunoreactivity for basic calponin was detected in the cytoplasm of 6 synovial sarcomas (43% positive). In the basic calponin-positive tumors and the HS-SY-II cells, expression for smooth muscle-specific genes, including basic calponin and SM22alpha, was detected by RT-PCR, suggesting a lineage relationship between synovial sarcoma cells and smooth muscle-like mesenchymal cells. Conclusions. A subset of synovial sarcomas expressing the basic calponin gene and the gene product were identified. The basic calponin may have potential utility as a novel molecular marker identifying certain synovial sarcomas.