Author： Ginkel Robert J. Van Berlo Charles L. H. Van Baas Peter C. Koops Heimen Schraffordt Stuling Ries Van Groningen B. Elstrodt Jan Hoekstra1 Harald J.
Publisher： Informa Healthcare
ISSN： 1369-1643
Source： Sarcoma, Vol.3, Iss.2, 1999-09, pp. : 89-94
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
Purpose. The feasibility of hyperthermic isolated limb perfusion (HILP) with tumor necrosis factor-alpha (TNFalpha) and cisplatin for the management of osteosarcoma was studied in the canine model. Methods. During seven perfusions in six healthy mongrel dogs (weight 32+/-2 kg) technical aspects of HILP under mild hyperthermia (39-40 C) were studied. In five experiments HILP was performed with TNFalpha alone (0.5 mg/l extremity volume), and in two experiments TNFalpha was combined with cisplatin (25 mg/l extremity volume). During the perfusions physiological parameters were monitored and TNFalpha and total cisplatin concentrations were determined. Results. Perfusion conditions (pH, PCO2, PO2, flow and pressure) remained within physiological ranges. Three dogs died within 24h despite a sublethal systemic concentration of TNFalpha that leaked from the perfusion circuit. Three dogs were terminated; one dog after the second experiment in accordance with Dutch ethical rules; one dog showed an invagination of the small bowel resulting in an ileus; one dog because of necrosis of the perfused limb. Conclusions. This feasibility study in healthy dogs demonstrated that HILP with TNFalpha and cisplatin was associated with a high mortality rate and does not allow us to treat dogs with spontaneous osteosarcoma with TNFalpha and cisplatin HILP. Therefore, an alternative model should be used in the search for the ideal combination of perfusion agents for limb sparing treatment in human osteosarcoma.
Related content
By Boesch Cedric E. Meyer Thomas Waschke Lena Merkel Susanne Goehl Jonas Hohenberger Werner Knorr Christian
International Journal of Hyperthermia, Vol. 26, Iss. 1, 2010-06 ,pp. :
Access to resources
Recommend
By Moller Mecker Lewis James Dessureault Sophie Zager Jonathan
International Journal of Hyperthermia, Vol. 24, Iss. 3, 2008-01 ,pp. :
By Walgenbach S. Bermes A. Bermes U. Bittinger F. Junginger Th. Mueller-Klieser W.
International Journal of Hyperthermia, Vol. 15, Iss. 2, 1999-03 ,pp. :