Author: Reiss E. Obayashi T. Orle K. Yoshida M. Zancopé-Oliveira R. M.
Publisher: Informa Healthcare
ISSN: 1460-2709
Source: Medical Mycology, Vol.38, Iss.1, 2000-12, pp. : 147-159
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Abstract
Non-culture methods being developed and evaluated for mycotic infections include polymerase chain reaction (PCR), galactomannan (GM) antigenemia, Western blot (WB) to detect antibodies, and detection of the fungal metabolites D-arabinitol and (1,3)-β-D-glucan. Sample preparation for PCR from blood specimens depends on fractionation of peripheral blood, its pre-incubation in blood culture broth, or a total DNA method, which does not rely on fractionation, or pre-incubation. Targets for PCR of fungi in the 18S or ITS2 subunits of the ribosomal RNA genes facilitated the design of
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