Author： Hordejuk Renata Lobanov Nikolai Kicinska Anna Szewczyk Adam Dolowy Krzysztof
Publisher： Informa Healthcare
ISSN： 1464-5203
Source： Molecular Membrane Biology, Vol.21, Iss.5, 2004-09, pp. : 307-313
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Abstract
We report here that large conductance K + selective channel in adrenal chromaffin granules is controlled by pH. We measured electrogenic influx of 86 Rb + into chromaffin granules prepared from bovine adrenal gland medulla. The 86 Rb + influx was inhibited by acidic pH. Purified chromaffin granule membranes were also fused with planar lipid bilayer. A potassium channel with conductance of 432±9 pS in symmetric 450 mM KCl was observed after reconstitution into lipid bilayer. The channel activity was unaffected by charybdotoxin, a blocker of the Ca 2+ -activated K + channel of large conductance. It was observed that acidification to pH 6.4 cis side of the membrane lowered the channel open probability and single channel conductance. Whereas only weak influence on the single channel current amplitude and open probability were observed upon lowering of the pH at the trans side. We conclude that a pH-sensitive large conductance potassium channel operates in the chromaffin granule membrane.
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