Insulin-loaded PLGA microparticles: flow focusing versus double emulsion/solvent evaporation

Author: Cóózar-Bernal M. J.   Holgado M. A.   Arias J. L.   Muññoz-Rubio I.   Martíín-Banderas L.   ÁÁlvarez-Fuentes J.   Fernáández-Aréévalo M.  

Publisher: Informa Healthcare

ISSN: 1464-5246

Source: Journal of Microencapsulation, Vol.28, Iss.5, 2011-08, pp. : 430-441

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Abstract

Context: Oral administration of insulin is severely limited by very low bioavailability. Biocompatible polymeric nanocarriers have been investigated to overcome this problem. Flow focusing (FF) has revolutionized current engineering of poly(D,L-lactide-co-glycolide) (PLGA) based micromedicines. This technique has never been used to formulate insulin-loaded PLGA microparticles.Objective: Investigation of the benefits rising from the synthesis of insulin-loaded PLGA microplatforms by FF, compared to double emulsion/solvent evaporation method.Materials and methods: Both synthesis methodologies were compared in terms of geometry, surface physicochemical properties and insulin vehiculization capabilities. The stability of the peptide during the formulation procedure was further analysed.Results: FF permitted the preparation of insulin-loaded microcarriers with better geometry and physicochemical properties for the oral route, along with greater insulin loading capabilities and sustained insulin release kinetics.Discussion and conclusion: Results have lead to the identification of the best formulation conditions for the engineering of insulin-loaded PLGA microparticles against diabetes.

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