Author： Peakman Luke J. Szczelkun Mark D.
Publisher： Oxford University Press
ISSN： 1362-4962
Source： Nucleic Acids Research, Vol.37, Iss.12, 2009-07, pp. : 3934-3945
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Abstract
In the absence of the methyl donor S-adenosyl methionine and under certain permissive reaction conditions, EcoPI shows non-specific endonuclease activity. We show here that the cofactor analogue S-adenosyl homocysteine promotes this promiscuous DNA cleavage. Additionally, an extensive exonuclease-like processing of the DNA is also observed that can even result in digestion of non-specific DNA in trans. We suggest a model for how DNA communication events initiating from non-specific sites, and in particular free DNA ends, could produce the observed cleavage patterns.
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