Author： Gong Seung Pyo Kim Heebal Lee Eun Ju Lee Seung Tae Moon Sunjin Lee Ho-Joon Lim Jeong Mook
Publisher： Oxford University Press
ISSN： 1460-2350
Source： Human Reproduction, Vol.24, Iss.4, 2009-04, pp. : 805-814
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Abstract
BACKGROUND We previously established parthenogenetic mouse embryonic stem cells (ESCs) and this study was subsequently conducted for elucidating the influence of oocyte parthenogenesis on gene expression profile of ESCs. METHODS Gene expression of parthenogenetic ESC (pESC)-1 or pESC-2 was separately compared with that of two normally fertilized ESC (nfESC) lines (B6D2F1 and R1 strains), and quantification of mRNA expression was conducted for validating microarray data. RESULTS In two sets of comparison, reaction of 11 347 and 15 454 gene probes were altered by parthenogenesis, while strain difference changed the expression of 15 750 and 14 944 probes. Level of correlation coefficient was higher in the comparisons between normal fertilization and parthenogenesis (0.9740.985) than in the comparisons between strains of nfESCs (0.970.971). Overall, the expression of 32763329 genes was changed after parthenogenesis, and 88% (96/109) of major functional genes differentially (P < 0.01)="" expressed="" in="" one="" comparison="" set="" showed="" the="" same="" change="" in="" the="" other.="" when="" we="" monitored="" imprinted="" genes,="" expression="" of="" nine="" paternal="" and="" eight="" maternal="" genes="" were="" altered="" after="" parthenogenesis="" and="" 88%="" (14/16)="" of="" these="" was="" confirmed="" by="" mrna=""> CONCLUSIONS The change in gene expression after parthenogenesis was similar to, or less than, the change induced by a strain difference under a certain genetic background. These results may suggest the clinical feasibility of parthenogenesis-derived, pluripotent cells.
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