Against the rules: A marine bacterium, Loktanella rosea , possesses a unique lipopolysaccharide

Author： Ieran Teresa Silipo Alba Nazarenko Evgeny L. Gorshkova Raisa P. Ivanova Elena P. Garozzo Domenico Sturiale Luisa Lanzetta Rosa Parrilli Michelangelo Molinaro Antonio

Publisher： Oxford University Press

ISSN： 1460-2423

Source： Glycobiology, Vol.20, Iss.5, 2010-05, pp. : 586-593

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Abstract

Bacteria are an inimitable source of new glyco-structures potentially useful in medicinal and environmental chemistry. Lipopolysaccharides (LPS; endotoxins) are the major components of the outer membrane of Gram-negative bacteria; being exposed toward the external environment they can undergo structural changes and thus, they often possess peculiar chemical features that allow them to thrive in harsh chemical and physical environments. Marine bacteria have evolved and adapted over millions of years in order to succeed in different environments, finding a niche for their survival characterized by severe physical or chemical parameters. The present work focuses on the structural investigation of the LPS from Loktanella rosea, a marine Gram-negative bacterium. Through chemical analysis, 2D nuclear magnetic resonance and matrix-assisted laser desorption ionization mass spectrometry investigations, a unique LPS carbohydrate backbone has been defined. The lipid A skeleton consists of a trisaccharide backbone lacking the typical phosphate groups and is characterized by two -glucosamines and an -galacturonic acid. The core region is built up of three ulosonic acids, with two 3-deoxy-d-manno-oct-2-ulopyranosonic acid residues, one of which is carrying a neuraminic acid. This carbohydrate structure is an exceptional variation from the typical architectural skeleton of endotoxins which consequently implies a very different biosynthesis.