Determination of Cocaine and Benzoylecgonine by Derivatization with Iodomethane-D 3 or PFPA/HFIP in Human Blood and Urine Using GC/MS (EI or PCI Mode)

Author： Aderjan Rolf E. Schmitt Georg Wu Moutian Meyer Christine

Publisher： Oxford University Press

ISSN： 0146-4760

Source： Journal of Analytical Toxicology, Vol.17, Iss.1, 1993-01, pp. : 51-55

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Abstract

A method for the simultaneous detection and quantification of cocaine and benzoylecgonine from human blood and urine has been developed. Cocaine and benzoylecgonine were extracted from blood and urine with solid-phase columns containing C₁₈ packing material. Clean, emulsion-free extracts were obtained. The analytes were derivatized with PFPA/HFIP or iodomethane-D₃. The underivatized cocaine and derivatized benzoylecgonine were determined by GC/MS with positive chemical ionization (PCI) and the selected ion monitoring mode. Benzoylecgonine was derivatized with iodomethane-D₃ to cocaine-D₃. Both derivatives were chromatographed on a CPSIL 5 capillary column. The recovery of extraction was greater than 70%. The detection limit for the PFPA/HFIP derivatives (20 ng/mL) was better than that for iodomethane-D₃ derivatives (40 ng/mL). The procedure is simple, rapid, and reproducible. Standard curves were linear and the procedure was suitable for routine analysis. Using fluorescence polarization Immunoassay (TDx, Abbott) (cutoff 100 ng/mL), along with one of the GC/MS methods described, we found no false positive or false negative results.