The Defined HHS/DOT Substituted Urine Criteria Validated Through a Controlled Hydration Study

ISSN： 0146-4760

Source： Journal of Analytical Toxicology, Vol.26, Iss.7, 2002-10, pp. : 419-423

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Abstract

This controlled hydration study was designed to validate the substitution criteria used by the Substance Abuse and Mental Health Services Administration (SAMHSA) to classify a workplace urine specimen as inconsistent with normal human urine. Study participants (n = 56) ingested at least 80 oz (2370 mL) of fluid over a 6-h period, 40 oz during the first 3 h (DOT hydration protocol) and 40 oz during the second 3-h period. Urine specimens (n = 498) were collected upon awakening, just prior to hydration, at the end of each hour of the 6-h test period, and upon awakening the next day. No urine specimen satisfied the paired substitution criteria of urine creatinine ≤ 5.0 mg/dL and specific gravity ≤ 1.001 or ≥ 1.020. Seventy-three percent of the participants produced at least one specimen meeting the criteria for dilute urine: urine creatinine < 20.0="" mg/dl="" and="" specific="" gravity="">< 1.003.="" fifty-five="" percent="" of="" the="" participants="" produced="" at="" least="" one="" dilute="" urine="" specimen="" during="" the="" first="" 3="" h="" of="" hydration.="" in="" conclusion,="" this="" controlled="" hydration="" study="" supports="" the="" criteria="" set="" by="" samhsa="" for="" classifying="" a="" specimen="" as="" substituted="" (inconsistent="" with="" normal="" human="" urine).="">