Bone Surface and Whole Bone as Biomarkers for Acute Fluoride Exposure

ISSN： 0146-4760

Source： Journal of Analytical Toxicology, Vol.29, Iss.8, 2005-11, pp. : 810-813

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Abstract

This study compares fluoride concentrations ([F]) in surface and whole bone for up to 27 days following an acute oral dose of F. Four groups of rats received single oral F dose (50 mg/kg body weight), and the control group received deionized water (n = 10/group). Groups were euthanized at 1, 3, 9, or 27 days after F administration. Plasma and femurs were collected. F on the femur surface was removed from a circular area (4.52 mm²) by immersion in 0.5M HCl for 15 s. The solution was buffered with total ionic strength adjustment buffer and analyzed with an electrode. The subjacent bone was sectioned and ashed at 600°C. Ash and plasma were analyzed for F with the electrode following hexamethyldisiloxane-facilitated diffusion. Data were analyzed by Kruskall-Wallis and Dunn's test and by linear regression (p < 0.05).="" peak="" plasma="" and="" bone="" surface="" [f]="" occurred="" on="" day="" 1="" (0.26="" ±="" 0.14="" µg/ml="" and="" 1801="" ±="" 888="" µg/g,="" respectively).="" bone="" surface="" [f]="" at="" 3,="" 9,="" and="" 27="" were="" not="" statistically="" different="" from="" control.="" a="" significant="" increase="" in="" whole="" bone="" [f]="" was="" observed="" 3="" days="" after="" f="" administration="" and="" the="" [f]="" remained="" relatively="" constant="" thereafter.="" the="" mean="" (±="" sd)="" surface/whole="" bone="" [f]="" ratios="" for="" the="" control="" and="" f="" groups="" were="" 2.45="" ±="" 0.98,="" 3.92="" ±="" 1.32,="" 1.61="" ±="" 0.82,="" 1.73="" ±="" 0.39,="" and="" 1.09="" ±="" 0.28,="" respectively.="" plasma="" and="" bone="" surface="" [f]s="" were="" positively="" correlated="" (r="0.74)." thus,="" bone="" surface="" was="" found="" to="" be="" a="" suitable="" biomarker="" for="" acute,="" sublethal="" f="" exposure="" 1="" day="" after="" f="" administration.="" whole="" bone="" [f]="" were="" significantly="" increased="" at="" 3,="" 9,="" and="" 27="" days="" after="" f="" administration.="">