Author： Scurlock Rodger D. Ohlson Greg B. Worthen David K.
Publisher： Oxford University Press
ISSN： 0146-4760
Source： Journal of Analytical Toxicology, Vol.30, Iss.4, 2006-05, pp. : 262-266
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Abstract
A method is described for the simultaneous analysis of Δ9-tetrahydrocannabinol (THC) and its carboxylic acid metabolite, 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THCA) as their trimethylsiyl derivatives using 2-dimensional chromatography and electron ionization-mass spectrometric detection. The addition of a Deans switch to a standard GC oven allows the use of two chromatographic columns of differing stationary phase to greatly reduce matrix interference. The analytes are extracted from 1 mL of whole blood by first precipitating the blood proteins with the addition of acetonitrile followed by solid-phase extraction. The limit of quantitation for both THC and THCA was determined to be 1.0 ng/mL. The between-run precision at 1.0 ng/mL (N = 30) was 7.7% and 7.4% for THC and THCA, respectively. The method is linear from 1 to 100 ng/mL.
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