SHORT COMMUNICATION: Ultra-Performance Liquid Chromatography– Tandem Mass Spectrometry Method for the Determination of Bupropion and its Main Metabolites in Human Whole Blood

Author： Denooz Raphaël

Publisher： Oxford University Press

ISSN： 0146-4760

Source： Journal of Analytical Toxicology, Vol.34, Iss.5, 2010-06, pp. : 280-286

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Abstract

A selective and sensitive ultra-performance liquid chromatography (UPLC)–electrospray ionization-tandem mass spectrometry (MS) method for simultaneous determination of bupropion and its main metabolites, hydroxybupropion, erythrohydrobupropion, and threohydrobupropion, in human whole blood is presented. The sample preparation consists of cleanup protein precipitation with methanol combined with a solid-phase extraction on Oasis HLB cartridges. Analytes were separated on a Waters Acquity UPLC^® BEH phenyl column using a binary mobile phase consisting of ammonium formate buffer (2 mM, pH 4) and acetonitrile. Detection was performed on a Waters Acquity UPLC system coupled to a Quattro Premier triple-quadrupole MS in positive ion selected reaction monitoring. Internal standards were bupropion-d₉ and hydroxybupropion-d₆. Linearity was from 5 to 1000 ng/mL for bupropion and from 10 to 2000 ng/mL for metabolites. Accuracy profiles (80–120%), precision (< 15%),="" and="" limits="" of="" detection="" (1="" ng/ml="" for="" bupropion="" and="" 2="" ng/ml="" for="" metabolites)="" were="" also="" evaluated="" and="" responded="" to="" all="" criteria="" of="" validation.="" the="" aim="" of="" this="" study="" was="" to="" compare="" this="" presented="" method="" with="" a="" previously="" described="" method="" developed="" on="" a="" classic="" liquid="" chromatography–tandem="" ms="" system.="">