Author： Cai Ming
Publisher： Springer Publishing Company
ISSN： 1610-1979
Source： The Chinese-German Journal of Clinical Oncology, Vol.7, Iss.6, 2008-06, pp. : 344-347
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
To construct siRNA recombinant expression vector targeting Livin gene and observe the apoptosis induction effect of it in human colon cancer cells.SiRNA recombinant expression vector targeting Livin gene was constructed and transfected into human colon cancer cell. The effect of siRNA recombinant expression vector was detected by RT-PCR, Western blot, MTT reduction assay and flow cytometry.It was confirmed by restriction endonuclease and sequence analysis that siRNA recombinant expression vector targeting Livin gene was constructed successfully. Inhibition ratio of Livin siRNA at mRNA and protein levels were 30.18% and 28.88%. The growth of cancer cells was inhibited significantly and the apoptotic ratio was 13.36 ± 1.45%.The siRNA recombinant expression vector targeting Livin gene has been constructed successfully. It not only can inhibit the expression of Livin gene but also can induce apoptosis in human colon cancer cells remarkably.
Related content
Access to resources
Recommend
By Zhou Dong Zhang Yi Liang Daoming Yuan Yong Zeng Demiao Chen Jiayong Yang Jie
Oncology Letters, Vol. 10, Iss. 6, 2015-12 ,pp. :