Measurement of Rabbit Apolipoprotein B by Use of Electroimmunodiffusion and Immunonephelometric Assays

Author： Tailleux Anne Labbe Julien Mallet Céline Percevault Frédéric Fruchart Jean-Charles Fievet Catherine

Publisher： American Association for Laboratory Animal Science

ISSN： 1532-0820

Source： Comparative Medicine, Vol.50, Iss.3, 2000-06, pp. : 309-313

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Abstract

Background and Purpose: Because of their similarity to humans, rabbits are a good animal model for the study of atherosclerosis associated with high serum low-density lipoprotein (LDL) values. Two assays were developed to measure apolipoprotein B (apoB), the major structural and functional apolipoprotein of LDL in rabbits and to distinguish endogenous LDL in transgenic rabbits from that of human apoB.Methods: Two procedures, an electroimmunoassay (EIA) and an immunonephelometric assay (INA), along with a goat-origin rabbit antiserum were developed to measure serum apoB concentration in rabbits.Results: Use of either assay resulted in ability to measure rabbit species-specific apoB concentration.Conclusion: These assays should have broad applications: to screen compounds or diets that might lower serum apoB concentrations; to specifically measure human apoB concentration in transgenic rabbits; to measure serum apoB concentration in rabbits overexpressing other human proteins.