Author： Estes Frances L. Myers Albert L. Briney Sharon
Publisher： Society for Applied Spectroscopy
ISSN： 0003-7028
Source： Applied Spectroscopy, Vol.24, Iss.1, 1970-01, pp. : 131-132
Disclaimer: Any content in publications that violate the sovereignty, the constitution or regulations of the PRC is not accepted or approved by CNPIEC.
Abstract
An analytical procedure utilizing infrared techniques has been developed for the quantitative determination of glutamic and α-ketoglutaric acids for samples containing milligram quantities of these acids. The infrared spectra of the samples in KBr pellets was determined on a Beckman IR-8 infrared spectrophotometer using double beam, slow scan, gains of 5.2% to 5.6% and attenuation to 97% T at 3846.2 cm−1. For preparation of the pellets, approximately 400 mg of KBr (IR grade, E. Merck, Darmstadt, Germany), was used. Better dispersion of the sample was achieved by mixing a part of the KBr with the sample in an agate mortar and combining this mixture with the rest of the KBr in the capsule of a Wig-L-Bug (Crescent Dental Mfg. Co., Chicago, Ill.) along with a nylon ball (⅛ in.). Twenty seconds agitation completed the mixing. The mixture was transferred to the die and the pellet prepared in the usual manner.
Related content
Access to resources
Recommend
By Gresham Christopher A. Gilmore Daniel A. Denton M. Bonner
Applied Spectroscopy, Vol. 53, Iss. 10, 1999-10 ,pp. :
By Holden Carol A. Hunnicutt Sally S. Sánchez-Ponce Raymundo Craig John M. Rutan Sarah C.
Applied Spectroscopy, Vol. 57, Iss. 5, 2003-05 ,pp. :
By Le Cacheux P. Ménard G. Quang H. Nguyen Weinmann P. Jouan M. Dao Nguyen Quy
Applied Spectroscopy, Vol. 50, Iss. 10, 1996-10 ,pp. :