Author: Thompson J.E. Jordan D.B.
Publisher: Elsevier
ISSN: 0003-2697
Source: Analytical Biochemistry, Vol.256, Iss.1, 1998-02, pp. : 7-13
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Abstract
We describe a multiwavelength method for measuring an enzyme's discrimination of one substrate over another by continuously monitoring the reactions of the two substrates simultaneously. This method is generally applicable to ultraviolet–visible diode array or rapid-scanning spectrophotometers and the measurement requires a single incubation of enzyme with two substrates. Rates at each of the wavelengths may be fit globally by using a nonlinear least-squares fitting procedure which provides adequate statistical analysis. The specificity of trypsin for
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