Author： Berestovskaya N.G. Shaloiko L.A. Gorokhovatsky A.Y. Bondar V.S. Vysotski E.S. Maximov J.E. von Doehren H. Alakhov Y.B.
Publisher： Elsevier
ISSN： 0003-2697
Source： Analytical Biochemistry, Vol.268, Iss.1, 1999-03, pp. : 72-78
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Abstract
Translation of apoobelin mRNA in a cell-free wheat germ translation system in the presence of coelenterazine and molecular oxygen results in cotranslational formation of active photoprotein. Active obelin formation is recorded by its luminescence, either direct in the translation mixture in the presence of coelenterazine and calcium ions or in aliquots from the translation mixture. In the second case translation is carried out with coelenterazine and EGTA. Registration of the translation course by luminescence of the synthesized product in both cases allows use of apoobelin mRNA at very low concentrations as an internal marker for immediate measure of protein biosynthesis activity of
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