Simultaneous Determination of Endogenous and Orally Administered 15N-Labeled Polyamines in Rat Organs

Author: Xu Y.J.   Hara T.   Samejima K.   Sasaki H.   Kobayashi M.   Takahashi A.   Niitsu M.  

Publisher: Elsevier

ISSN: 0003-2697

Source: Analytical Biochemistry, Vol.301, Iss.2, 2002-02, pp. : 255-260

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Abstract

A method for the simultaneous determination of polyamines (putrescine, spermidine, and spermine) by ionspray ionization-mass spectrometry was modified to determine 15N-labeled polyamines together with unlabeled polyamines using 13C,15N double-labeled polyamines as internal standards. This technique permitted the use of 15N-labeled polyamines as tracer compounds to follow polyamine biosynthesis, interconversion, and absorption. The method was used to examine the organ distribution of orally administered 15N-labeled polyamines in rats. Each 15N-labeled polyamine was taken up by the three organs tested: the small intestine, liver, and kidney. The uptake of 15N-labeled spermidine was greater than that of 15N-labeled spermine and putrescine. Administration of a mixture of 15N-labeled polyamines was useful for determining the disposition of each 15N-polyamine absorbed from the intestinal tract. ©2002 Elsevier Science (USA).