Author: Ahn T. Kim J-S. Lee B-C. Yun C-H.
Publisher: Elsevier
ISSN: 0003-9861
Source: Archives of Biochemistry and Biophysics, Vol.395, Iss.1, 2001-11, pp. : 14-20
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Abstract
The effects of nonlamellar-prone lipids, diacylglycerol and phosphatidylethanolamine (PE), on the kinetic association of SecA with model membranes were examined by measuring changes in the intrinsic emission fluorescence with a stopped-flow apparatus. Upon interaction with standard liposomes composed of 50 mol% dioleolyphosphatidylcholine (DOPC) and 50 mol% of dioleoylphosphatidylglycerol (DOPG), the intrinsic fluorescence intensity of SecA was decreased after a lapse of time with a rate constant of 0.0049 s-1. When the DOPC of the standard vesicles was gradually replaced with either dioeloyl PE (DOPE) or
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