A Novel Monoclonal Antibody to N -Myristoyl Glycine Moiety Found a New N -Myristoylated HIV-1 p28 gag Protein in HIV-1-Infected Cells

Author： Furuishi K. Misumi S. Shoji S.

Publisher： Elsevier

ISSN： 0006-291X

Source： Biochemical and Biophysical Research Communications, Vol.222, Iss.2, 1996-05, pp. : 344-351

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Abstract

A novel monoclonal antibody was raised against a synthetic N -myristoyl glycine that is characteristic of all N -myristoylated proteins. The immunoreaction suppressed in the presence of hemocyanin as well as albumin conjugated with N -myristoyl glycine and other N -myristoyl glycyl peptides, while underivatized and myristoyl amino acid proteins or various fatty acids other than myristic acid exerted no effect. The antibody specifically reacted with N -myristoylated pp60 c-src in human colon adenocarcinoma cells, N -myristoylated pp60 v-src in Rous sarcoma virus-infected cells, and N -myristoylated Gag precursor protein Pr55 gag in HIV-1-producing cells. Furthermore, the antibody immunoreacted with a new N -myristoylated p28 gag derived from HIV-1 gag protein. The antibody is shown to be a very useful tool for identification of N -myristoylated proteins.